Chromatography

Background Chlorophyll often hides the other pigments present in leaves. In autumn, chlorophyl breaks down, allowing xanthophyll and carotene, and newly made anthocyanin, to point their colors. The liquify of pigments in a leaf may be illogical into bands of color by the technique of typography chromatography. Chromatography involves the insularity of varietys into somebody components. Chromatography means color writing. With this technique the components of a mixture in a liquid medium are spaced. The legal separation takes orchestrate by engrossment and capillarity. The paper holds the substances by absorption; capillarity pulls the substances up the paper at different rates. Pigments are separated on the paper and show up as dark streaks. The pattern of separated components on the paper is called a chromatogram. Question Which pigments underside you identify in a jet-propelled plane leaf? Prediction The pigments that ask on on the chromatograph paper shoul d be related to pigments seen on plants in real life. extinct of various possibilities, I believe that trine pigments that may be disc all overed in this lab allow be carotene, chlorophyll a, and xanthrophylls. Safety Precautions The solvent is volatile. Ensure that in that respect are no flames in the classroom, and avoid breathing vapors from the solvent. The classroom must be well-ventilated Materials * Spinach leaves * Solvent (90% rock crude ether and 10% acetone) * Chromatography paper * Paper clip * return stand * Test-tube clamp * Cork stopper * come aft(prenominal) glass * Large evidence tube Procedure 1. hold fast the large test tube to a retort stand. 2. model up the bobsleigh stopper and the paper clip as shown. 3. Measure a piece of chromatography paper so that it is ache enough to go down from the paper clip only when not so long that it touches the bottom of the test tube. (Refer to the diagrams). twist the paper to a advert! at one end. 4. flummox a coleus or spinach leaf over the pointed end of the chromatography...If you want to get a full essay, ball club it on our website: BestEssayCheap.com

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